Regulatory

Part:BBa_K4061000:Design

Designed by: Diya Agrawal   Group: iGEM21_HKUST   (2021-09-23)


OmpF promoter, controlled by OmpR regulatory protein as part of the Two Component System in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

OmpF promoter has 4 regulatory regions- F1, F2, F3 are positive regulators while F4 is a negative regulator. Binding of phosphorylated OmpR modulates gene expression, the positive regulatory units have a higher affinity to OmpR while negative regulator has a lower affinity comparitively. The coding sequence and essential regions were confirmed with "Transcription Regulation of ompF and ompC by a Single Transcription Factor, OmpR" by Yoshida et al (2006).


Source

Escherichia Coli (Strain K12), 500bp upstream from the gene OMPF. The coding sequence is oriented in a direction opposite to the chromosome, hence obtained the +500 base pair sequence.


References