Part:BBa_K4061000:Design
OmpF promoter, controlled by OmpR regulatory protein as part of the Two Component System in E. coli
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
OmpF promoter has 4 regulatory regions- F1, F2, F3 are positive regulators while F4 is a negative regulator. Binding of phosphorylated OmpR modulates gene expression, the positive regulatory units have a higher affinity to OmpR while negative regulator has a lower affinity comparitively. The coding sequence and essential regions were confirmed with "Transcription Regulation of ompF and ompC by a Single Transcription Factor, OmpR" by Yoshida et al (2006).
Source
Escherichia Coli (Strain K12), 500bp upstream from the gene OMPF. The coding sequence is oriented in a direction opposite to the chromosome, hence obtained the +500 base pair sequence.